For the more mathematically minded, formulas are presented below for percent reduction calculations, log reduction calculations, and finally a formula is presented which will allow people to go back and forth between the two. Breadcrumb Home Information.

So, if a surface begins with 1,000 microbes on it, a 3-log reduction would reduce the number of microorganisms to 1. Read more. This figure expresses the probability of a single item in a batch being non-sterile after being subjected to a sterilization process.

So, a UV light that manages somehow to get a 2-log kill on an array of one million C.

An effective process has a very low SAL, meaning that there is an extremely small likelihood of any infecting microbes surviving the sterilization. The easiest way to consider the difference is that SAL is the probability of there being a non-sterile unit and log reduction is the percentage of microbes eliminated.

Colony Forming Unit: A more complete list of microbes and their dosage requirements can be found in this application note. Similarly, when calculating and reporting the changes in CFUs after disinfection, rather than state the magnitude of change in individual CFUs, microbiologists express the performance as a percentage reduction in terms of a reduction factor and, for convenience, typically in factors of 10 using a logarithmic log reduction scale — a log reduction factor LRV.

However, because of the way log scales work, a 2. If you ever have any questions about a study report from our lab or any other commercial microbiology lab, we are glad to help you understand it. Dosage is determined based on the intensity of the UVC energy and the exposure time at a specific wavelength. The EPA allows products that meet their standards to be called sporicides, the equivalent to what the FDA calls sterilants.

According to the CDC, healthcare-acquired C.

To assess the effectiveness of any sterilization process, scientists use a unit of measure called sterility assurance level, or SAL. So how are log rates calculated?

For example, a 1 log reduction corresponds to inactivating 90 percent of a target microbe with the microbe count being reduced by a factor of 10. For instance, to say your product kills 99.

Instead, by diluting a sample and spreading this across a petri plate, microbiologists can count groups of microbes, called colonies. The Halo Disinfection System , on the other hand, produces an odor-free and non-corrosive mist that has been validated by the EPA to achieve a 6-log kill of C.

Hopefully this page has been helpful.

Privacy Policy. Similarly, when calculating the magnitute of the change in cell number, microbiologists often use a logarithmic scale log scale.

The Halo Disinfection System kills 6-logs. SAL of 10-6 indicates a 1 in 1,000,000 likelihood of an organism surviving to the end of the sterilization process.

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